Species determination within Staphylococcus genus by extended PCR-restriction fragment length polymorphism of saoC gene

FEMS Microbiol Lett. 2015 Jan;362(1):1-11. doi: 10.1093/femsle/fnu007. Epub 2014 Dec 4.

Abstract

Genetic methods based on PCR-restriction fragment length polymorphism (RFLP) are widely used for microbial species determination. In this study, we present the application of saoC gene as an effective tool for species determination and within-species diversity analysis for Staphylococcus genus. The unique sequence diversity of saoC allows us to apply four restriction enzymes to obtain RFLP patterns, which appear highly distinctive even among closely related species as well as atypical isolates of environmental origin. Such patterns were successfully obtained for 26 species belonging to Staphylococcus genus. What is more, tracing polymorphisms detected by different restriction enzymes allowed for basic phylogeny analysis for Staphylococcus aureus, which is potentially applicable for other staphylococcal species.

Keywords: Staphylococcus aureus; Staphylococcus phylogeny; coagulase-negative staphylococci; genotyping; species determination; typing methods.

Publication types

  • Evaluation Study
  • Research Support, Non-U.S. Gov't

MeSH terms

  • Bacterial Proteins / genetics*
  • Genetic Variation*
  • Genotype
  • Humans
  • Molecular Typing / methods*
  • Polymerase Chain Reaction*
  • Polymorphism, Restriction Fragment Length*
  • Staphylococcus / classification*
  • Staphylococcus / genetics*

Substances

  • Bacterial Proteins