Description of bovine major histocompatibility complex class IIa haplotypes using parthenogenetic embryo-derived cells

A L A Sá; V S G Leal; M P C Schneider; O M Ohashi; E J M Santos; P K Riggs; K J Kochan; C A Gill; E D Downey; J E Womack; L C Skow; M S Miranda; L Sena

doi:10.1111/age.12285

Description of bovine major histocompatibility complex class IIa haplotypes using parthenogenetic embryo-derived cells

Anim Genet. 2015 Jun;46(3):325-8. doi: 10.1111/age.12285. Epub 2015 Mar 19.

Authors

A L A Sá¹, V S G Leal, M P C Schneider, O M Ohashi, E J M Santos, P K Riggs, K J Kochan, C A Gill, E D Downey, J E Womack, L C Skow, M S Miranda, L Sena

Affiliation

¹ Laboratório de Fecundação In Vitro, Instituto de Ciências Biológicas, Universidade Federal do Pará, Belém, PA, 66075-110, Brazil.

PMID: 25789691
DOI: 10.1111/age.12285

Abstract

In this study, we report an approach to characterize individual BoLA haplotypes using cells from parthenogenetic bovine embryos derived from slaughterhouse ovaries. Eight of the 15 parthenogenetic embryos so obtained had not undergone meiotic recombination on the BoLA region and were suitable to describe BoLA haplotypes. Detailed analysis of the BoLA class IIa region identified seven different class IIa haplotypes, including six not previously described and two new alleles of BoLA-DQA and one BoLA-DQB. Our method provided reliable sources of homozygous DNA to describe BoLA haplotypes.

Keywords: BoLA haplotypes; DRB3-DQA-DQB; MHC; oocyte activation.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alleles
Animals
Cattle / genetics*
Embryo, Mammalian
Genes, MHC Class II*
Haplotypes*
Parthenogenesis