Easy to use plastic optical fiber-based biosensor for detection of butanal

Nunzio Cennamo; Stefano Di Giovanni; Antonio Varriale; Maria Staiano; Fabio Di Pietrantonio; Andrea Notargiacomo; Luigi Zeni; Sabato D'Auria

doi:10.1371/journal.pone.0116770

Easy to use plastic optical fiber-based biosensor for detection of butanal

PLoS One. 2015 Mar 19;10(3):e0116770. doi: 10.1371/journal.pone.0116770. eCollection 2015.

Authors

Nunzio Cennamo¹, Stefano Di Giovanni², Antonio Varriale², Maria Staiano², Fabio Di Pietrantonio³, Andrea Notargiacomo⁴, Luigi Zeni¹, Sabato D'Auria⁵

Affiliations

¹ Department of Industrial and Information Engineering, SUN, Aversa, Italy.
² Institute of Protein Biochemistry, CNR, Napoli, Italy.
³ "O.M.Corbino" Institute of Acoustics and Sensors, Rome, Italy.
⁴ Institute for Photonics and Nanotechnologies, CNR, Rome, Italy.
⁵ Institute of Protein Biochemistry, CNR, Napoli, Italy; Institute of Food Science, CNR, Avellino, Italy.

Abstract

The final goal of this work is to achieve a selective detection of butanal by the realization of a simple, small-size and low cost experimental approach. To this end, a porcine odorant-binding protein was used in connection with surface plasmon resonance transduction in a plastic optical fiber tool for the selective detection of butanal by a competitive assay. This allows to reduce the cost and the size of the sensing device and it offers the possibility to design a "Lab-on-a-chip" platform. The obtained results showed that this system approach is able to selectively detect the presence of butanal in the concentration range from 20 μM to 1000 μM.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Aldehydes / analysis*
Aldehydes / chemistry
Animals
Biosensing Techniques / methods*
Optical Fibers*
Receptors, Odorant / chemistry*
Surface Plasmon Resonance / methods*
Sus scrofa

Substances

Aldehydes
Receptors, Odorant
odorant-binding protein
butyraldehyde

Grants and funding

This project was realized within the CNR Commessa “Progettazione e Sviluppo di Biochip per la Sicurezza Alimentare e Salute Umana”, the CISIA project and the Cluster CLAN “Safe&Smart”. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.