Receptor usage and cell entry of porcine epidemic diarrhea coronavirus

Chang Liu; Jian Tang; Yuanmei Ma; Xueya Liang; Yang Yang; Guiqing Peng; Qianqian Qi; Shibo Jiang; Jianrong Li; Lanying Du; Fang Li

doi:10.1128/JVI.00430-15

Receptor usage and cell entry of porcine epidemic diarrhea coronavirus

J Virol. 2015 Jun;89(11):6121-5. doi: 10.1128/JVI.00430-15. Epub 2015 Mar 18.

Authors

Chang Liu¹, Jian Tang², Yuanmei Ma³, Xueya Liang³, Yang Yang¹, Guiqing Peng⁴, Qianqian Qi⁵, Shibo Jiang⁵, Jianrong Li³, Lanying Du⁶, Fang Li⁷

Affiliations

¹ Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA.
² Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA.
³ Department of Veterinary Biosciences, College of Veterinary Medicine, The Ohio State University, Columbus, Ohio, USA.
⁴ State Key Laboratory of Agricultural Microbiology, College of Veterinary Medicine, Huazhong Agricultural University, Wuhan, Hubei, China.
⁵ Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA Key Laboratory of Medical Molecular Virology of Ministries of Education and Health, Shanghai Medical College and Institute of Medical Microbiology, Fudan University, Shanghai, China.
⁶ Lindsley F. Kimball Research Institute, New York Blood Center, New York, New York, USA ldu@nybloodcenter.org lifang@umn.edu.
⁷ Department of Pharmacology, University of Minnesota Medical School, Minneapolis, Minnesota, USA ldu@nybloodcenter.org lifang@umn.edu.

Abstract

Porcine epidemic diarrhea coronavirus (PEDV) has significantly damaged America's pork industry. Here we investigate the receptor usage and cell entry of PEDV. PEDV recognizes protein receptor aminopeptidase N from pig and human and sugar coreceptor N-acetylneuraminic acid. Moreover, PEDV infects cells from pig, human, monkey, and bat. These results support the idea of bats as an evolutionary origin for PEDV, implicate PEDV as a potential threat to other species, and suggest antiviral strategies to control its spread.

Publication types

Research Support, N.I.H., Extramural

MeSH terms

Animals
CD13 Antigens / metabolism*
Chiroptera
Haplorhini
Humans
N-Acetylneuraminic Acid / metabolism*
Porcine epidemic diarrhea virus / physiology*
Receptors, Coronavirus
Receptors, Virus / metabolism*
Swine
Virus Attachment*
Virus Internalization*

Substances

Receptors, Coronavirus
Receptors, Virus
CD13 Antigens
N-Acetylneuraminic Acid

Abstract

Publication types

MeSH terms

Substances

Grants and funding