Effective saccharification of kraft pulp by using a cellulase cocktail prepared from genetically engineered Aspergillus oryzae

Biosci Biotechnol Biochem. 2015;79(6):1034-7. doi: 10.1080/09168451.2015.1006568. Epub 2015 Mar 18.

Abstract

Kraft pulp is a promising feedstock for bioproduction. The efficiency of kraft pulp saccharification was improved by using a cellulase cocktail prepared from genetically engineered Aspergillus oryzae. Application of the cellulase cocktail was demonstrated by simultaneous saccharification and fermentation, using kraft pulp and non-cellulolytic yeast. Such application would make possible to do an efficient production of other chemicals from kraft pulp.

Keywords: Aspergillus oryzae; cellulase; fermentation; kraft pulp; simultaneous saccharification.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aspergillus oryzae / genetics*
  • Aspergillus oryzae / metabolism*
  • Cellulase / metabolism*
  • Cellulose / chemistry*
  • Fermentation
  • Genetic Engineering*
  • Hydrolysis
  • Plasmids / genetics
  • Wood / chemistry

Substances

  • Cellulose
  • Cellulase