Three-wavelength light control of freely moving Drosophila Melanogaster for less perturbation and efficient social-behavioral studies

Yen-Yin Lin; Ming-Chin Wu; Po-Yen Hsiao; Li-An Chu; Mei-Mei Yang; Chien-Chung Fu; Ann-Shyn Chiang

doi:10.1364/BOE.6.000514

Three-wavelength light control of freely moving Drosophila Melanogaster for less perturbation and efficient social-behavioral studies

Biomed Opt Express. 2015 Jan 13;6(2):514-23. doi: 10.1364/BOE.6.000514. eCollection 2015 Feb 1.

Authors

Yen-Yin Lin¹, Ming-Chin Wu², Po-Yen Hsiao³, Li-An Chu⁴, Mei-Mei Yang⁵, Chien-Chung Fu⁶, Ann-Shyn Chiang⁷

Affiliations

¹ Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Photonics Technologies, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Electrical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
² Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
³ Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan ; These authors contributed equally to this work.
⁴ Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan.
⁵ Department of Art and Creative Design, Hsuan Chuang University, Hsinchu 30013, Taiwan.
⁶ Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Department of Power Mechanical Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Nanotechnology and Microsystems Engineering, National Tsing Hua University, Hsinchu 30013, Taiwan ; ccfu@mx.nthu.edu.tw.
⁷ Brain Research Center, National Tsing Hua University, Hsinchu 30013, Taiwan ; Institute of Biotechnology, National Tsing Hua University, Hsinchu 30013, Taiwan ; Genomics Research Center, Academia Sinica, Nankang, Taipei 11529, Taiwan ; Kavli Institute for Brain and Mind, University of California, San Diego, La Jolla, CA 92093-0526, USA.

Abstract

We developed a real-time automated laser-tracking system combined with continuous wave 1064-nm infrared or 473-nm blue lasers to provide punishment for studying memory in Drosophila Melanogaster. Combining optogenetic tools with laser properties, such as 473-nm and 593-nm lasers that activate light sensitive proteins in artificial transgenic flies, we can manipulate the specific neuron of an assigned fly among multiple flies to investigate neuron circuit relationships in social interactions. In restraining condition assay or optogenetic experiments, a ventral irradiated system would be more efficient due to higher ventral cuticle transmissions and neuron ganglia locations. Therefore, ventral irradiated systems cause less perturbation during behavior studies.

Keywords: (100.5010) Pattern recognition; (170.1420) Biology; (170.2655) Functional monitoring and imaging; (170.3660) Light propagation in tissues.