High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues

Chun-Yu Lin; Pei-Kao Li; Li-Chung Cheng; Yi-Cheng Li; Chia-Yuan Chang; Ann-Shyn Chiang; Chen Yuan Dong; Shean-Jen Chen

doi:10.1364/BOE.6.000491

High-throughput multiphoton-induced three-dimensional ablation and imaging for biotissues

Biomed Opt Express. 2015 Jan 12;6(2):491-9. doi: 10.1364/BOE.6.000491. eCollection 2015 Feb 1.

Authors

Chun-Yu Lin¹, Pei-Kao Li¹, Li-Chung Cheng², Yi-Cheng Li², Chia-Yuan Chang², Ann-Shyn Chiang³, Chen Yuan Dong⁴, Shean-Jen Chen⁵

Affiliations

¹ Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan.
² Department of Photonics, National Cheng Kung University, Tainan 701, Taiwan.
³ Brain Research Center, National Tsing Hua University, Hsinchu 300, Taiwan ; Institute of Biotechnology and Department of Life Science, National Tsing Hua University, Hsinchu 300, Taiwan ; aschiang@life.nthu.edu.tw.
⁴ Department of Physics, National Taiwan University, Taipei 106, Taiwan ; cydong@phys.ntu.edu.tw.
⁵ Department of Engineering Science, National Cheng Kung University, Tainan 701, Taiwan ; Center for Micro/Nano Science and Technology, National Cheng Kung University, Tainan 701, Taiwan ; Advanced Optoelectronic Technology Center, National Cheng Kung University, Tainan 701, Taiwan.

Abstract

In this study, a temporal focusing-based high-throughput multiphoton-induced ablation system with axially-resolved widefield multiphoton excitation has been successfully applied to rapidly disrupt biotissues. Experimental results demonstrate that this technique features high efficiency for achieving large-area laser ablation without causing serious photothermal damage in non-ablated regions. Furthermore, the rate of tissue processing can reach around 1.6 × 10(6) μm(3)/s in chicken tendon. Moreover, the temporal focusing-based multiphoton system can be efficiently utilized in optical imaging through iterating high-throughput multiphoton-induced ablation machining followed by widefield optical sectioning; hence, it has the potential to obtain molecular images for a whole bio-specimen.

Keywords: (140.3390) Laser materials processing; (170.1020) Ablation of tissue; (190.4180) Multiphoton processes.