Predominance of KPC-3 in a survey for carbapenemase-producing Enterobacteriaceae in Portugal

Antimicrob Agents Chemother. 2015;59(6):3588-92. doi: 10.1128/AAC.05065-14. Epub 2015 Mar 16.

Abstract

Among the 2,105 Enterobacteriaceae tested in a survey done in Portugal, 165 were nonsusceptible to carbapenems, from which 35 (26 Klebsiella pneumoniae, 3 Escherichia coli, 2 Enterobacter aerogenes, and 3 Enterobacter cloacae isolates and 1 Klebsiella oxytoca isolate) were confirmed to be carbapenemase producers by the presence of 30 Tn4401d-blaKPC-3, 4 intI3-blaGES-5, and one intI1-blaVIM-2 gene, alone or in combination with other bla genes. The dissemination of blaKPC-3 gene carried by an IncF plasmid suggests lateral gene transfer as a major mechanism of dissemination.

Publication types

  • Research Support, N.I.H., Extramural
  • Research Support, Non-U.S. Gov't
  • Research Support, U.S. Gov't, Non-P.H.S.

MeSH terms

  • Bacterial Proteins / genetics
  • Bacterial Proteins / metabolism*
  • Enterobacter aerogenes / enzymology
  • Enterobacteriaceae / enzymology*
  • Escherichia coli / enzymology
  • Klebsiella oxytoca / enzymology
  • Klebsiella pneumoniae / enzymology
  • Microbial Sensitivity Tests
  • Plasmids / genetics
  • Portugal
  • beta-Lactamases / genetics
  • beta-Lactamases / metabolism*

Substances

  • Bacterial Proteins
  • beta-Lactamases
  • carbapenemase