Evolution and single-nucleotide polymorphisms in methicillin-resistant Staphylococcus aureus strains with reduced susceptibility to vancomycin and daptomycin, based on determination of the complete genome

Tetsuo Yamaguchi; Shingo Suzuki; Sakiko Okamura; Yuri Miura; Ayaka Tsukimori; Itaru Nakamura; Norihiko Ito; Anri Masuya; Takashi Shiina; Tetsuya Matsumoto

doi:10.1128/AAC.05159-14

Evolution and single-nucleotide polymorphisms in methicillin-resistant Staphylococcus aureus strains with reduced susceptibility to vancomycin and daptomycin, based on determination of the complete genome

Antimicrob Agents Chemother. 2015;59(6):3585-7. doi: 10.1128/AAC.05159-14. Epub 2015 Mar 16.

Authors

Affiliations

¹ Department of Microbiology, Tokyo Medical University, Tokyo, Japan Department of Infection Control, Tokyo Medical University Hospital, Tokyo, Japan tetsuo.yamaguchi@med.toho-u.ac.jp.
² Department of Molecular Life Science, Tokai University School of Medicine, Kanagawa, Japan.
³ Department of Medical Treatment for Health Scientific Research, Bunkyo Gakuin University Graduate School, Tokyo, Japan.
⁴ Department of Microbiology Laboratory, Tokyo Medical University Hospital, Tokyo, Japan.
⁵ Department of Microbiology, Tokyo Medical University, Tokyo, Japan Department of Infection Control, Tokyo Medical University Hospital, Tokyo, Japan.
⁶ Department of Infection Control, Tokyo Medical University Hospital, Tokyo, Japan.
⁷ Center for Biological Resources and Informatics, Dainichiseika-Donated Chair of Research Division for Innovative Biomaterials, Tokyo Institute of Technology, Tokyo, Japan Department of Ophthalmology, Tokyo Medical University Hospital, Tokyo, Japan.

Abstract

We obtained a series of methicillin-resistant Staphylococcus aureus isolates, including both daptomycin-susceptible strain TD1 and daptomycin-resistant strain TD4, from a patient. We determined the complete genome sequences of TD1 and TD4 using next-generation sequencing, and only four single-nucleotide polymorphisms (SNPs) were identified, one each in capB (E58K), rpoB (H481Y), lytN (I16V), and mprF (V351E). We determined that these four SNPs were sufficient to cause the strains to develop daptomycin, vancomycin, and rifampin resistance.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Biological Evolution*
Daptomycin / pharmacology*
Gene Expression Regulation, Bacterial / genetics
Methicillin-Resistant Staphylococcus aureus / drug effects*
Methicillin-Resistant Staphylococcus aureus / genetics*
Microbial Sensitivity Tests
Molecular Sequence Data
Polymorphism, Single Nucleotide / genetics
Vancomycin / pharmacology*

Substances

Anti-Bacterial Agents
Vancomycin
Daptomycin

Associated data

GENBANK/AP014652
GENBANK/AP014653