An aryl-thioether substituted nitrobenzothiadiazole probe for the selective detection of cysteine and homocysteine

Chem Commun (Camb). 2015 Apr 18;51(30):6518-20. doi: 10.1039/c5cc01071c.

Abstract

An aryl-thioether substituted nitrobenzothiadiazole probe was synthesized and employed to detect cysteine and homocysteine selectively in living cells. Interestingly, both cysteine (Cys) and homocysteine (Hcy) promote an enhancement of the fluorescence intensity of the probe at pH 7.4 while only Cys gives rise to this enhancement under weakly acidic conditions (pH 6.0).

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Cysteine / analysis*
  • Cysteine / chemistry*
  • Fluorescent Dyes / chemistry*
  • HeLa Cells
  • Homocysteine / analysis*
  • Homocysteine / chemistry*
  • Humans
  • Hydrogen-Ion Concentration
  • Sulfides / chemistry*

Substances

  • Fluorescent Dyes
  • Sulfides
  • Homocysteine
  • Cysteine