Development of a novel in vitro onychomycosis model for the evaluation of topical antifungal activity

Reindert Sleven; Ellen Lanckacker; Gaëlle Boulet; Peter Delputte; Louis Maes; Paul Cos

doi:10.1016/j.mimet.2015.03.009

Development of a novel in vitro onychomycosis model for the evaluation of topical antifungal activity

J Microbiol Methods. 2015 May:112:73-5. doi: 10.1016/j.mimet.2015.03.009. Epub 2015 Mar 12.

Authors

Reindert Sleven¹, Ellen Lanckacker¹, Gaëlle Boulet¹, Peter Delputte¹, Louis Maes¹, Paul Cos²

Affiliations

¹ Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium.
² Laboratory of Microbiology, Parasitology and Hygiene (LMPH), Faculty of Pharmaceutical, Biomedical and Veterinary Sciences, University of Antwerp, Universiteitsplein 1, B-2610 Antwerp, Belgium. Electronic address: paul.cos@uantwerpen.be.

PMID: 25772040
DOI: 10.1016/j.mimet.2015.03.009

Abstract

A novel in vitro onychomycosis model was developed to easily predict the topical activity potential of novel antifungal drugs. The model encompasses drug activity and diffusion through bovine hoof slices in a single experimental set-up. Results correspond well with the antifungal susceptibility assay and Franz cell diffusion test.

Keywords: Drug evaluation; Onychomycosis; Topical antifungals.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Infective Agents, Local / pharmacokinetics
Anti-Infective Agents, Local / pharmacology*
Antifungal Agents / pharmacokinetics
Antifungal Agents / pharmacology*
Cattle
Drug Evaluation, Preclinical / methods*
Hoof and Claw / drug effects
Hoof and Claw / microbiology
Models, Theoretical
Onychomycosis / drug therapy*

Substances

Anti-Infective Agents, Local
Antifungal Agents