Abstract
As a surrogate of live cells, proteo-lipobeads are presented, encapsulating functional membrane proteins in a strict orientation into a lipid bilayer. Assays can be performed just as on live cells, for example using fluorescence measurements. As a proof of concept, we have demonstrated proton transport through cytochrome c oxidase.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Biomimetics
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Electron Transport Complex IV / chemistry*
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Fluorescence
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Fluorescent Dyes / chemistry
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Lipid Bilayers / chemistry*
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Sepharose / chemistry*
Substances
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Fluorescent Dyes
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Lipid Bilayers
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Sepharose
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Electron Transport Complex IV