Identifying Haemophilus haemolyticus and Haemophilus influenzae by SYBR Green real-time PCR

Roger Latham; Bowen Zhang; Stephen Tristram

doi:10.1016/j.mimet.2015.03.001

Identifying Haemophilus haemolyticus and Haemophilus influenzae by SYBR Green real-time PCR

J Microbiol Methods. 2015 May:112:67-9. doi: 10.1016/j.mimet.2015.03.001. Epub 2015 Mar 6.

Authors

Roger Latham¹, Bowen Zhang², Stephen Tristram³

Affiliations

¹ School of Medicine, University of Tasmania, Hobart, Tasmania, Australia. Electronic address: Roger.Latham@utas.edu.au.
² School of Health Sciences, University of Tasmania, Launceston, Australia. Electronic address: Bowen.Zhang@utas.edu.au.
³ School of Health Sciences, University of Tasmania, Launceston, Australia. Electronic address: Stephen.Tristram@utas.edu.au.

PMID: 25753676
DOI: 10.1016/j.mimet.2015.03.001

Abstract

SYBR Green real time PCR assays for protein D (hpd), fuculose kinase (fucK) and [Cu, Zn]-superoxide dismutase (sodC) were designed for use in an algorithm for the identification of Haemophilus influenzae and H. haemolyticus. When tested on 127 H. influenzae and 60 H. haemolyticus all isolates were identified correctly.

Keywords: Clinical microbiology; Differentiation; Haemophilus haemolyticus; Haemophilus influenzae; Identification; PCR; Real-time PCR.

Publication types

Evaluation Study

MeSH terms

Bacterial Proteins / genetics
Haemophilus / classification
Haemophilus / genetics
Haemophilus / isolation & purification*
Haemophilus Infections / diagnosis
Haemophilus Infections / microbiology
Humans
Molecular Diagnostic Techniques / methods*
Real-Time Polymerase Chain Reaction / methods*

Substances

Bacterial Proteins