Abstract
We have determined a 1.18 Å resolution X-ray crystal structure of a novel ruthenocenyle-6-aminopenicillinic acid in complex with CTX-M β-lactamase, showing unprecedented details of interactions between ruthenocene and protein. As the first product complex with an intact catalytic serine, the structure also offers insights into β-lactamase catalysis and inhibitor design.
Publication types
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Research Support, N.I.H., Extramural
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Research Support, Non-U.S. Gov't
MeSH terms
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Anti-Bacterial Agents / chemical synthesis
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Anti-Bacterial Agents / chemistry
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Anti-Bacterial Agents / pharmacology*
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Crystallography, X-Ray
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Enterococcus / drug effects*
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Microbial Sensitivity Tests
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Models, Molecular
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Molecular Conformation
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Organometallic Compounds / chemistry
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Organometallic Compounds / pharmacology*
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Staphylococcus / drug effects*
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beta-Lactamases / metabolism
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beta-Lactams / chemistry
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beta-Lactams / pharmacology*
Substances
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Anti-Bacterial Agents
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Organometallic Compounds
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beta-Lactams
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ruthenocene
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beta-Lactamases