A search for restriction fragment length polymorphism on the human Y chromosome

Hum Genet. 1989 Dec;84(1):86-8. doi: 10.1007/BF00210680.

Abstract

A systematic search for restriction fragment length polymorphisms (RFLPs) on the human Y chromosome was performed. DNA samples from 16-34 individuals were screened with five restriction enzymes and 12 Y-chromosomal probes, 3 of which detect lowly repetitive sequences and 9 of which are apparently single copy in genomic DNA. None of the single-copy probes revealed any variation. The repetitive sequence probe p21A1 (DYZ?) revealed a TaqI RFLP with q = 0.05. The frequency of fixed point mutations in Y-chromosomal DNA outside the pseudoautosomal region is probably less than 1 in 18,000 bp.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Blotting, Southern
  • DNA / genetics
  • DNA Probes
  • DNA Restriction Enzymes
  • Deoxyribonucleases, Type II Site-Specific
  • Female
  • Humans
  • Male
  • Pedigree
  • Polymorphism, Restriction Fragment Length*
  • Y Chromosome*

Substances

  • DNA Probes
  • DNA
  • DNA Restriction Enzymes
  • Deoxyribonucleases, Type II Site-Specific
  • TCGA-specific type II deoxyribonucleases