Seed oil polyphenols: rapid and sensitive extraction method and high resolution-mass spectrometry identification

Abstract

Phenolic content is a primary parameter for vegetables oil quality evaluation, and directly involved in the prevention of oxidation and oil preservation. Several methods have been reported in the literature for polyphenols extraction from seed oil but the approaches commonly used remain manually handled. In this work, we propose a rapid and sensitive method for seed oil polyphenols extraction and identification. For this purpose, polyphenols were extracted from Opuntia stricta Haw seed oil, using high frequency agitation, separated, and then identified using a liquid chromatography-high resolution mass spectrometry method. Our results showed good sensitivity and reproducibility of the developed methods.

Keywords: HPLC–HRMS; Opuntia stricta Haw; Seed oil phenolic compounds; Supercritical CO(2).