Rapid Decoding of Sequence-Specific Nuclease-Induced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR Products

Xingliang Ma; Letian Chen; Qinlong Zhu; Yuanling Chen; Yao-Guang Liu

doi:10.1016/j.molp.2015.02.012

Rapid Decoding of Sequence-Specific Nuclease-Induced Heterozygous and Biallelic Mutations by Direct Sequencing of PCR Products

Mol Plant. 2015 Aug;8(8):1285-7. doi: 10.1016/j.molp.2015.02.012. Epub 2015 Mar 5.

Authors

Xingliang Ma¹, Letian Chen², Qinlong Zhu³, Yuanling Chen⁴, Yao-Guang Liu⁵

Affiliations

¹ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
² State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; Guangdong Provincial Key Laboratory of Protein Function and Regulation in Agricultural Organisms, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
³ Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
⁴ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China.
⁵ State Key Laboratory for Conservation and Utilization of Subtropical Agro-Bioresources, Guangzhou 510642, China; Key Laboratory of Plant Functional Genomics and Biotechnology of Guangdong Provincial Higher Education Institutions, Guangzhou 510642, China; College of Life Sciences, South China Agricultural University, Guangzhou 510642, China. Electronic address: ygliu@scau.edu.cn.

PMID: 25747846
DOI: 10.1016/j.molp.2015.02.012

No abstract available

Publication types

Letter
Research Support, Non-U.S. Gov't

MeSH terms

Alleles*
Base Sequence
Deoxyribonucleases / metabolism*
Heterozygote
Homozygote
Molecular Sequence Data
Mutation / genetics*
Polymerase Chain Reaction / methods*
Sequence Analysis, DNA / methods*

Substances

Deoxyribonucleases