First case of NDM-1 producing Klebsiella pneumoniae in Caribbean islands

Sylvaine Bastian; Patrice Nordmann; Elodie Creton; Edith Malpote; Guillaume Thiery; Frederic Martino; Sebastien Breurec; Laurent Dortet

doi:10.1016/j.ijid.2015.03.002

First case of NDM-1 producing Klebsiella pneumoniae in Caribbean islands

Int J Infect Dis. 2015 May:34:53-4. doi: 10.1016/j.ijid.2015.03.002. Epub 2015 Mar 5.

Authors

Sylvaine Bastian¹, Patrice Nordmann², Elodie Creton³, Edith Malpote⁴, Guillaume Thiery⁵, Frederic Martino⁴, Sebastien Breurec⁵, Laurent Dortet⁶

Affiliations

¹ University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France. Electronic address: sylvaine.bastian@chu-guadeloupe.fr.
² INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France; Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France; University of Fribourg, Fribourg, Switzerland; Hôpital Fribourgeois - Hôpital Cantonal, Fribourg, Switzerland.
³ INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France; Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France.
⁴ University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France.
⁵ University Hospital of Pointe-à-Pitre/Abymes, Pointe-à-Pitre, Guadeloupe, France; University of Antilles, Pointe-à-Pitre, Guadeloupe, France.
⁶ INSERM U914 « Emerging Resistance to Antibiotic », Le Kremlin-Bicêtre, France; Associated National Reference Center to Antibiotic Resistance, Le Kremlin-Bicêtre, France; South Paris University, Faculty of Medecine, Le Kremlin-Bicêtre, France; Assistance Publique des Hôpitaux de Paris, Bicêtre Hospital, Le Kremlin-Bicêtre, France.

PMID: 25747780
DOI: 10.1016/j.ijid.2015.03.002

Abstract

Objectives: Characterize a NDM-1 producing K. pneumoniae isolate recovered from a patient hospitalized in Guadeloupe, French West Indies, after its transfer from Cuba

Methods: Antibiotic susceptibilities were determined by the disk diffusion method, and E-test. Carbapenemase production was assessed using the Carba NP test. Antibiotic resistance determinants and their surrounding structures were characterized by PCR mapping and DNA sequencing. Transfer of the β-lactam resistance marker was attempted by liquid mating-out assays

Results: Here we reported the first NDM-1 producing enterobacterial isolate recovered from Caribbean islands. This K. pneumoniae isolate belongs to a new sequence type (ST1649). The blaNDM-1 gene together with the aacA4 gene were carried on a self conjugative IncR plasmid of c.a. 80kb.

Conclusion: This study describes the first identification of a NDM-1 producer in Caribbean islands. The uncommon incompatibility group of the blaNDM-1 carrying plasmid and the uncommon ST type of the K. pneumoniae strain suggest a possible local emergence of NDM producers.

Keywords: Cuba; Guadeloupe; NDM-1; carbapenemase; metallo-β-lactamase.

Publication types

Case Reports
Research Support, Non-U.S. Gov't

MeSH terms

Aged
Anti-Bacterial Agents / pharmacology*
Anti-Bacterial Agents / therapeutic use
Bacterial Proteins / genetics
Cuba
Guadeloupe
Humans
Klebsiella Infections / diagnosis*
Klebsiella Infections / drug therapy
Klebsiella Infections / microbiology
Klebsiella pneumoniae / drug effects
Klebsiella pneumoniae / genetics
Klebsiella pneumoniae / isolation & purification*
Male
Microbial Sensitivity Tests
Plasmids / genetics
Plasmids / isolation & purification
Polymerase Chain Reaction
Travel
beta-Lactam Resistance / genetics
beta-Lactamases / genetics*
beta-Lactams / pharmacology*
beta-Lactams / therapeutic use

Substances

Anti-Bacterial Agents
Bacterial Proteins
beta-Lactams
beta-Lactamases
beta-lactamase NDM-1
carbapenemase