A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out

Robert J Meier; Johann M B Simbürger; Tero Soukka; Michael Schäferling

doi:10.1039/c5cc00144g

A FRET based pH probe with a broad working range applicable to referenced ratiometric dual wavelength and luminescence lifetime read out

Chem Commun (Camb). 2015 Apr 11;51(28):6145-8. doi: 10.1039/c5cc00144g.

Authors

Robert J Meier¹, Johann M B Simbürger, Tero Soukka, Michael Schäferling

Affiliation

¹ Institute of Analytical Chemistry, Chemo- and Biosensors, University of Regensburg, 93040 Regensburg, Germany.

PMID: 25747771
DOI: 10.1039/c5cc00144g

Abstract

A luminescent probe for determination of pH was designed based on a Förster resonance energy transfer (FRET) system, combining a europium chelate as the donor and carboxynaphtho-fluorescein as a pH sensitive acceptor. The FRET system enables referenced pH detection in an exceptional broad dynamic range from pH 3 to 9.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Chelating Agents / chemistry
Europium / chemistry
Fluorescence Resonance Energy Transfer*
Fluorescent Dyes / chemical synthesis
Fluorescent Dyes / chemistry*
Hydrogen-Ion Concentration
Luminescence*
Molecular Structure

Substances

Chelating Agents
Fluorescent Dyes
Europium