Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells

Xiyun Ruan; Zhenguo Yuan; Yifeng Du; Guangxiao Yang; Quanying Wang

doi:10.3969/j.issn.1673-5374.2012.09.012

Recombinant adeno-associated virus delivered human thioredoxin-PR39 prevents hypoxia-induced apoptosis of ECV304 cells

Neural Regen Res. 2012 Mar 25;7(9):708-13. doi: 10.3969/j.issn.1673-5374.2012.09.012.

Authors

Xiyun Ruan¹, Zhenguo Yuan², Yifeng Du¹, Guangxiao Yang³, Quanying Wang³

Affiliations

¹ Department of Neurology, Shandong Provincial Hospital Affiliated to Shandong University, Jinan 250021, Shandong Province, China.
² Shandong Medical Imaging Research Institute, Affiliated to Shandong University, Jinan 250021, Shandong Province, China.
³ Xi'an Huaguang Biological Engineering Co., Ltd., Xi'an 710025, Shaanxi Province, China.

PMID: 25745468
PMCID: PMC4347013
DOI: 10.3969/j.issn.1673-5374.2012.09.012

Abstract

Human thioredoxin and antibacterial peptide, PR39, have been shown to have potent antioxidant effects that may prolong survival of cells during hypoxia. The pSSCMV/human thioredoxin-PR39 vector was successfully constructed in this study and used to infect ECV304 cells. Transfected ECV304 cells were incubated at 1%, 5% hypoxic, and normal oxygen conditions. We found that the number of apoptotic cells after transfection with recombinant adeno-associated virus-human thioredoxin -PR39 was significantly lower than controls, suggesting a protective effect of the recombinant human thioredoxin-PR39 protein on hypoxic cells.

Keywords: antimicrobial peptide PR39; apoptosis; fusion gene; gene therapy; human thioredoxin; hypoxia; recombinant adeno-associated virus.