The p110α isoform of phosphoinositide 3-kinase is essential for cone photoreceptor survival

Raju V S Rajala; Michelle Ranjo-Bishop; Yuhong Wang; Ammaji Rajala; Robert E Anderson

doi:10.1016/j.biochi.2015.02.018

The p110α isoform of phosphoinositide 3-kinase is essential for cone photoreceptor survival

Biochimie. 2015 May:112:35-40. doi: 10.1016/j.biochi.2015.02.018. Epub 2015 Mar 3.

Authors

Raju V S Rajala¹, Michelle Ranjo-Bishop², Yuhong Wang², Ammaji Rajala², Robert E Anderson³

Affiliations

¹ Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Department of Physiology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA. Electronic address: raju-rajala@ouhsc.edu.
² Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.
³ Department of Ophthalmology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Department of Cell Biology, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA; Dean A. McGee Eye Institute, University of Oklahoma Health Sciences Center, Oklahoma City, OK 73104, USA.

Abstract

Phosphoinositide 3-kinases (PI3Ks) are a family of lipid kinases that phosphorylates the 3'OH of the inositol ring of phosphoinositides (PIs). They are responsible for coordinating a diverse range of cellular functions. Class IA PI3K is a heterodimeric protein composed of a regulatory p85 and a catalytic p110 subunit. In this study, we conditionally deleted the p110α-subunit of PI3K in cone photoreceptor cells using the Cre-loxP system. Cone photoreceptors allow for color vision in bright light (daylight vision). Cone-specific deletion of p110α resulted in cone degeneration. Our studies suggest that PI3K signaling is essential for cone photoreceptor functions.

Keywords: Cone photoreceptors; Cre-loxP system; Phosphoinositide 3-kinases; Phosphoinositides; Retina; Vision.

Publication types

Research Support, N.I.H., Extramural
Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Survival / physiology
Class I Phosphatidylinositol 3-Kinases / genetics
Class I Phosphatidylinositol 3-Kinases / metabolism*
Mice
Mice, Knockout
Retinal Cone Photoreceptor Cells / cytology
Retinal Cone Photoreceptor Cells / enzymology*
Signal Transduction / physiology*

Substances

1-phosphatidylinositol 3-kinase p110 subunit, mouse
Class I Phosphatidylinositol 3-Kinases

Abstract

Publication types

MeSH terms

Substances

Grants and funding