Improved production of reducing sugars from rice straw using crude cellulase activated with Fe₃O₄/alginate nanocomposite

Neha Srivastava; Jay Singh; Pramod W Ramteke; P K Mishra; Manish Srivastava

doi:10.1016/j.biortech.2015.02.059

Improved production of reducing sugars from rice straw using crude cellulase activated with Fe₃O₄/alginate nanocomposite

Bioresour Technol. 2015 May:183:262-6. doi: 10.1016/j.biortech.2015.02.059. Epub 2015 Feb 20.

Authors

Neha Srivastava¹, Jay Singh², Pramod W Ramteke³, P K Mishra⁴, Manish Srivastava⁵

Affiliations

¹ Department of Biotechnology, Allahabad Agriculture University (ICAR), Allahabad 211007, India; Department of Chemical Engineering, Indian Institute of Technology (IIT), Banaras Hindu University, Varanasi 221005, India.
² Department of Applied Chemistry, Delhi Technological University, Shahbad Daulatpur, Main Bawana Road, Delhi 110042, India.
³ Department of Biotechnology, Allahabad Agriculture University (ICAR), Allahabad 211007, India.
⁴ Department of Chemical Engineering, Indian Institute of Technology (IIT), Banaras Hindu University, Varanasi 221005, India.
⁵ Department of Physics & Astrophysics, University of Delhi, Delhi 110007, India. Electronic address: 84.srivastava@gmail.com.

PMID: 25740000
DOI: 10.1016/j.biortech.2015.02.059

Abstract

Effect of Fe3O4 nanoparticles (NPs) and Fe3O4/Alginate nanocomposites (NCs) have been investigated on production and thermostability of crude cellulase enzyme system obtained by newly isolated thermotolerant Aspergillus fumigatus AA001. Fe3O4 NPs and Fe3O4/Alginate NCs have been synthesized by co-precipitation method and characterized through various techniques. In presence of Fe3O4 NPs and Fe3O4/Alginate NCs, filter paper activity of crude cellulase was increased about 35% and 40%, respectively in 72 h as compared to control. Fe3O4/Alginate NCs treated crude enzyme was thermally stable up to 8h at 70°C and retained 56% of its relative activity whereas; control samples could retain only 19%. Further, the hydrolysis of 1.0% alkali treated rice straw using Fe3O4/Alginate NCs treated cellulase gave much higher sugar productivity than control at optimal condition. These findings may be utilized in the area of biofuels and biowaste management.

Keywords: Cellulases; Fe(3)O(4) nanoparticle; Saccharification; Sugar productivity; Thermostability.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Alginates / pharmacology*
Aspergillus fumigatus / isolation & purification
Aspergillus fumigatus / metabolism
Carbohydrates / biosynthesis*
Cellulase / metabolism*
Enzyme Activation / drug effects
Enzyme Stability / drug effects
Ferric Compounds / pharmacology*
Glucuronic Acid / pharmacology
Hexuronic Acids / pharmacology
Hydrolysis
Nanocomposites / chemistry*
Nanocomposites / ultrastructure
Oryza / chemistry*
Oxidation-Reduction
Spectroscopy, Fourier Transform Infrared
Temperature
Waste Products*
X-Ray Diffraction

Substances

Alginates
Carbohydrates
Ferric Compounds
Hexuronic Acids
Waste Products
ferric oxide
Glucuronic Acid
Cellulase