Combining a sensor and a pH-gated nanopore based on an avidin-biotin system

Chem Commun (Camb). 2015 Apr 7;51(27):5994-7. doi: 10.1039/c4cc10087e. Epub 2015 Mar 5.

Abstract

Here we propose a new approach to tailor nanopores, which combines both pH gating and sensing properties. This strategy is based on PEG like-avidin grafting in nanopores designed by atomic layer deposition (ALD). Below pH 5 the nanopore is blocked. We show that the PEG chains are at the origin of these properties.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Avidin / chemistry*
  • Biosensing Techniques*
  • Biotin / chemistry*
  • Biotinylation
  • Carbodiimides / chemistry
  • Cross-Linking Reagents / chemistry
  • Electrochemical Techniques
  • Humans
  • Hydrogen-Ion Concentration
  • Nanoparticles / chemistry*
  • Nanopores
  • Polyethylene Glycols / chemistry
  • Solutions
  • gamma-Globulins / analysis*

Substances

  • 1-ethyl-3-(3-(diethylamino)propyl)carbodiimide
  • Carbodiimides
  • Cross-Linking Reagents
  • Solutions
  • gamma-Globulins
  • Avidin
  • Polyethylene Glycols
  • Biotin