Abstract
Selective targeting of tumor cells by nanoparticle-based drug delivery systems is highly desirable because it maximizes the drug concentration at the desired target while simultaneously protecting the surrounding healthy tissues. Here, we show a design for smart nanocarriers based on a biomimetic approach that utilizes the building principle of virus envelope structures. Emulsomes and CurcuEmulsomes comprising a tripalmitin solid core surrounded by phospholipid layers are modified by S-layer proteins that self-assemble into a two-dimensional array to form a surface layer. One significant advantage of this nanoformulation is that it increases the solubility of the lipophilic anti-cancer agent curcumin in the CurcuEmulsomes by a factor of 2700. In order to make the emulsomes specific for IgG, the S-layer protein is fused with two protein G domains. This S-layer fusion protein preserves its recrystallization characteristics, forming an ordered surface layer (square lattice with 13 nm unit-by-unit distance). The GG domains are presented in a predicted orientation and exhibit a selective binding affinity for IgG.
Keywords:
Active drug delivery; Curcumin; Emulsomes; Immunoglobulin G (IgG) Targeting; S-layer (fusion) proteins.
Copyright © 2015 The Authors. Published by Elsevier B.V. All rights reserved.
Publication types
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Research Support, Non-U.S. Gov't
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Antineoplastic Agents, Phytogenic / chemistry*
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Bacillaceae / chemistry
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Bacterial Proteins / chemistry
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Bacterial Proteins / genetics
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Bacterial Proteins / metabolism
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Biomimetic Materials / chemistry
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Curcumin / chemistry*
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Drug Compounding
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Drug Delivery Systems*
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Emulsions
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Escherichia coli / genetics
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Escherichia coli / metabolism
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Gene Expression
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Humans
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Hydrophobic and Hydrophilic Interactions
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Immunoconjugates / chemistry
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Immunoconjugates / metabolism
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Immunoglobulin G / chemistry*
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Immunoglobulin G / metabolism
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Liposomes / chemistry
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Liposomes / metabolism
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Membrane Glycoproteins / chemistry*
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Membrane Glycoproteins / genetics
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Membrane Glycoproteins / metabolism
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Monosaccharide Transport Proteins / chemistry
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Monosaccharide Transport Proteins / genetics
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Monosaccharide Transport Proteins / metabolism
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Nucleocapsid / chemistry
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Protein Binding
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Protein Structure, Tertiary
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Recombinant Fusion Proteins / chemistry*
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Recombinant Fusion Proteins / genetics
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Recombinant Fusion Proteins / metabolism
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Solubility
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Triglycerides / chemistry
Substances
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Antineoplastic Agents, Phytogenic
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Bacterial Proteins
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Emulsions
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Immunoconjugates
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Immunoglobulin G
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Liposomes
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Membrane Glycoproteins
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Monosaccharide Transport Proteins
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Recombinant Fusion Proteins
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S-layer proteins
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SbpA protein, bacteria
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Triglycerides
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tripalmitin
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Curcumin