Agrobacterium-mediated transformation of tomato with the ICE1 transcription factor gene

Genet Mol Res. 2015 Jan 30;14(1):597-608. doi: 10.4238/2015.January.30.1.

Abstract

ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitative results showed that the ICE1 gene was transferred to tomato; three strains tested positive. After low-temperature stress treatment, praline content and peroxide and catalase activities in the transgenic tomato plants were higher compared with non-transgenic controls, while malondialdehyde content was clearly lower.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptation, Physiological / drug effects
  • Agrobacterium / drug effects
  • Agrobacterium / growth & development
  • Agrobacterium / physiology*
  • Anti-Bacterial Agents / pharmacology
  • Carbenicillin / pharmacology
  • Cold Temperature
  • Cotyledon / drug effects
  • Cotyledon / microbiology
  • Electrophoresis, Agar Gel
  • Genes, Plant*
  • Genetic Vectors / metabolism
  • Kanamycin / pharmacology
  • Plants, Genetically Modified
  • Polymerase Chain Reaction
  • Regeneration / drug effects
  • Solanum lycopersicum / drug effects
  • Solanum lycopersicum / genetics*
  • Solanum lycopersicum / microbiology*
  • Time Factors
  • Transcription Factors / genetics*
  • Transformation, Genetic* / drug effects

Substances

  • Anti-Bacterial Agents
  • ICE1 protein, Arabidopsis
  • Transcription Factors
  • Kanamycin
  • Carbenicillin