Abstract
ICE1 genes play a very important role in plants in cold conditions. To improve the cold resistance of tomato, the ICE1 gene of Arabidopsis thaliana was used to construct the plant expression vector p3301-ICE1, and was overexpressed in tomato through Agrobacterium-mediated transformation. Five strains of resistant plants were obtained. PCR and half-quantitative results showed that the ICE1 gene was transferred to tomato; three strains tested positive. After low-temperature stress treatment, praline content and peroxide and catalase activities in the transgenic tomato plants were higher compared with non-transgenic controls, while malondialdehyde content was clearly lower.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Adaptation, Physiological / drug effects
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Agrobacterium / drug effects
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Agrobacterium / growth & development
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Agrobacterium / physiology*
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Anti-Bacterial Agents / pharmacology
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Carbenicillin / pharmacology
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Cold Temperature
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Cotyledon / drug effects
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Cotyledon / microbiology
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Electrophoresis, Agar Gel
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Genes, Plant*
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Genetic Vectors / metabolism
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Kanamycin / pharmacology
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Plants, Genetically Modified
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Polymerase Chain Reaction
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Regeneration / drug effects
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Solanum lycopersicum / drug effects
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Solanum lycopersicum / genetics*
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Solanum lycopersicum / microbiology*
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Time Factors
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Transcription Factors / genetics*
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Transformation, Genetic* / drug effects
Substances
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Anti-Bacterial Agents
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ICE1 protein, Arabidopsis
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Transcription Factors
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Kanamycin
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Carbenicillin