Molecular cloning and characterization of autophagy-related gene TmATG8 in Listeria-invaded hemocytes of Tenebrio molitor

Dev Comp Immunol. 2015 Jul;51(1):88-98. doi: 10.1016/j.dci.2015.02.017. Epub 2015 Feb 26.

Abstract

Macroautophagy (hereinafter called autophagy) is a highly regulated process used by eukaryotic cells to digest portions of the cytoplasm that remodels and recycles nutrients and disposes of unwanted cytoplasmic constituents. Currently 36 autophagy-related genes (ATG) and their homologs have been characterized in yeast and higher eukaryotes, including insects. In the present study, we identified and functionally characterized the immune function of an ATG8 homolog in a coleopteran insect, Tenebrio molitor (TmATG8). The cDNA of TmATG8 comprises of an ORF of 363 bp that encodes a protein of 120 amino acid residues. TmATG8 transcripts are detected in all the developmental stages analyzed. TmAtg8 protein contains a highly conserved C-terminal glycine residue (Gly116) and shows high amino acid sequence identity (98%) to its Tribolium castaneum homolog, TcAtg8. Loss of function of TmATG8 by RNAi led to a significant increase in the mortality rates of T. molitor larvae against Listeria monocytogenes. Unlike dsEGFP-treated control larvae, TmATG8-silenced larvae failed to turn-on autophagy in hemocytes after injection with L. monocytogenes. These data suggest that TmATG8 play a role in mediating autophagy-based clearance of Listeria in T. molitor.

Keywords: ATG8; Autophagy; Innate immunity; Listeria monocytogenes; RNA interference; Tenebrio molitor.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adaptor Proteins, Signal Transducing / genetics
  • Amino Acid Sequence
  • Animals
  • Autophagy / genetics
  • Autophagy-Related Protein 8 Family
  • Bacterial Load / genetics
  • Cloning, Molecular
  • Hemocytes / microbiology
  • Hemocytes / physiology*
  • Listeria monocytogenes / immunology*
  • Listeriosis / immunology*
  • Microfilament Proteins / genetics
  • Microfilament Proteins / metabolism*
  • Molecular Sequence Data
  • RNA, Small Interfering / genetics
  • Sequence Alignment
  • Sequence Homology, Amino Acid
  • Tenebrio / immunology*

Substances

  • Adaptor Proteins, Signal Transducing
  • Autophagy-Related Protein 8 Family
  • GABARAPL2 protein, human
  • Microfilament Proteins
  • RNA, Small Interfering