In situ synthesis of homochiral metal-organic framework in capillary column for capillary electrochromatography enantioseparation

Congjie Pan; Weifeng Wang; Huige Zhang; Laifang Xu; Xingguo Chen

doi:10.1016/j.chroma.2015.02.034

In situ synthesis of homochiral metal-organic framework in capillary column for capillary electrochromatography enantioseparation

J Chromatogr A. 2015 Apr 3:1388:207-16. doi: 10.1016/j.chroma.2015.02.034. Epub 2015 Feb 16.

Authors

Congjie Pan¹, Weifeng Wang¹, Huige Zhang¹, Laifang Xu¹, Xingguo Chen²

Affiliations

¹ State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China.
² State Key Laboratory of Applied Organic Chemistry, Lanzhou University, Lanzhou 730000, China; Department of Chemistry, Lanzhou University, Lanzhou 730000, China; Key Laboratory of Nonferrous Metal Chemistry and Resources Utilization of Gansu Province, Lanzhou 730000, China. Electronic address: chenxg@lzu.edu.cn.

PMID: 25725957
DOI: 10.1016/j.chroma.2015.02.034

Abstract

Homochiral metal-organic frameworks (MOFs) are promising as porous stationary phase for open-tubular capillary electrochromatography (OT-CEC) enantioseparation owing to their fine-tuned pore sizes and large surface areas. In this work, the homochiral MOF AlaZnCl was successfully coated on the inner wall of fused silica capillary by an in situ, layer-by-layer self-assembly approach at room temperature. The results of scanning electron microscopy (SEM), X-ray diffraction (XRD), streaming potential (SP) and Fourier-transform infrared spectroscopy (FT-IR) indicated that the homochiral MOF AlaZnCl was successfully coated on the capillary inner wall. To evaluate the performance of the homochiral MOF AlaZnCl coated capillary column, the enantioseparation was carried out by using six amine drugs and monoamine neurotransmitters as model analytes and excellent enantioseparation efficiency was achieved. Run-to-run, day-to-day, and column-to-column relative standard deviations (RSDs) were all less than 5%. Moreover, the separation efficiency of the homochiral MOF AlaZnCl coated capillary column did not decrease obviously over 100 runs.

Keywords: Enantioseparation; Homochiral metal–organic framework; In situ synthesis; MOF AlaZnCl; Open tubular capillary electrochromatography.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Capillary Electrochromatography / methods*
Microscopy, Electron, Scanning / methods*
Organometallic Compounds / chemical synthesis*
Porosity
Silicon Dioxide / chemistry*
Spectroscopy, Fourier Transform Infrared / methods*
Stereoisomerism
X-Ray Diffraction / methods*

Substances

Organometallic Compounds
Silicon Dioxide