Abstract
A common feature of stress signalling pathways are alterations in the concentration of cytosolic free calcium ([Ca2+]cyt), which allow the specific and rapid transmission of stress signals through a plant after exposure to a stress, such as salinity. Here, we used an aequorin based bioluminescence assay to compare the NaCl-induced changes in [Ca2+]cyt of the Arabidopsis ecotypes Col-0 and C24. We show that C24 lacks the NaCl specific component of the [Ca2+]cyt signature compared to Col-0. This phenotypic variation could be exploited as a screening methodology for the identification of yet unknown components in the early stages of the salt signalling pathway.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Aequorin / genetics
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Aequorin / metabolism
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Arabidopsis / drug effects*
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Arabidopsis / genetics
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Arabidopsis / metabolism*
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Arabidopsis Proteins / genetics
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Arabidopsis Proteins / metabolism
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Calcium / metabolism*
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Cytosol / metabolism
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Ecotype
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Seedlings / genetics
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Seedlings / metabolism
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Signal Transduction / drug effects*
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Sodium Chloride / metabolism*
Substances
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Arabidopsis Proteins
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Sodium Chloride
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Aequorin
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Calcium
Grants and funding
The authors would like to thank the Australian Centre for Plant Functional Genomics, Australian Research Council, The Grains Research and Development Corporation, The Waite Research Institute, the Barr Smith Travel Scholarship in Agriculture, the Brenda Nettle Travel Award and King Abdullah University for Science and Technology for financial support. The funders had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.