Structure and genetics of the O-antigen of Enterobacter cloacae G3054 containing di-N-acetylpseudaminic acid

Andrei V Perepelov; Min Wang; Andrei V Filatov; Xi Guo; Alexander S Shashkov; Lei Wang; Yuriy A Knirel

doi:10.1016/j.carres.2015.01.015

Structure and genetics of the O-antigen of Enterobacter cloacae G3054 containing di-N-acetylpseudaminic acid

Carbohydr Res. 2015 Apr 30:407:59-62. doi: 10.1016/j.carres.2015.01.015. Epub 2015 Jan 31.

Authors

Andrei V Perepelov¹, Min Wang², Andrei V Filatov³, Xi Guo², Alexander S Shashkov³, Lei Wang⁴, Yuriy A Knirel³

Affiliations

¹ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation. Electronic address: perepel@ioc.ac.ru.
² TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China.
³ N. D. Zelinsky Institute of Organic Chemistry, Russian Academy of Sciences, 119991 Moscow, Russian Federation.
⁴ TEDA Institute of Biological Sciences and Biotechnology, Nankai University, TEDA, Tianjin 300457, China; Key Laboratory of Molecular Microbiology and Technology of the Ministry of Education, College of Life Sciences, Nankai University, Tianjin 300071, China; Tianjin Biochip Corporation, TEDA, Tianjin 300457, China.

PMID: 25723621
DOI: 10.1016/j.carres.2015.01.015

Abstract

Mild acid degradation of the lipopolysaccharide of Enterobacter cloacae G3054 resulted in the cleavage of the O-polysaccharide at the linkage of residues of 5,7-diacetamido-3,5,7,9-tetradeoxy-L-glycero-L-manno-non-2-ulosonic acid (di-N-acetylpseudaminic acid, Pse5Ac7Ac) in the main chain. The resultant oligosaccharide and an alkali-treated lipopolysaccharide were studied by sugar analysis along with (1)H and (13)C NMR spectroscopy, and the following structure of the branched pentasaccharide O-unit of the O-polysaccharide was established: [structure: see text] The O-antigen gene cluster of E. cloacae G3054 between conserved genes galF and gnd was sequenced. Most genes necessary for the O-antigen synthesis were found in the cluster and their functions were tentatively assigned by comparison with sequences in the available databases.

Keywords: Bacterial polysaccharide structure; Enterobacter cloacae; O-antigen; O-antigen gene cluster; Pseudaminic acid.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Carbohydrate Sequence
Enterobacter cloacae / genetics*
Enterobacter cloacae / metabolism
Lipopolysaccharides / chemistry
Multigene Family
O Antigens / chemistry*
O Antigens / genetics*
O Antigens / isolation & purification
Proton Magnetic Resonance Spectroscopy
Sialic Acids / chemistry*

Substances

5,7-diacetamido-3,5,7,9-tetradeoxynon-2-ulosonic acid
Lipopolysaccharides
O Antigens
Sialic Acids