Quantification of interferon signaling in avian cells

Joeri Kint; Maria Forlenza

doi:10.1007/978-1-4939-2438-7_21

Quantification of interferon signaling in avian cells

Methods Mol Biol. 2015:1282:251-9. doi: 10.1007/978-1-4939-2438-7_21.

Authors

Joeri Kint¹, Maria Forlenza

Affiliation

¹ Cell Biology and Immunology Group, Wageningen Institute of Animal Sciences, Wageningen University, Wageningen, The Netherlands.

Abstract

Activation of the type I interferon (IFN) response is an essential defense mechanism against invading pathogens such as viruses. This chapter describes two protocols to quantify activation of the chicken IFN response through analysis of gene expression by real-time quantitative PCR and by quantification of bioactive IFN protein using a bioassay.

MeSH terms

Animals
Avian Proteins / genetics
Avian Proteins / metabolism*
Cell Line
Chickens
Coronavirus / physiology*
Host-Pathogen Interactions*
Interferon Type I / genetics
Interferon Type I / metabolism*
Quail
RNA, Messenger / genetics
RNA, Messenger / isolation & purification
RNA, Messenger / metabolism
Signal Transduction
Transcription, Genetic

Substances

Avian Proteins
Interferon Type I
RNA, Messenger