Abstract
Rapamycin-induced protein heterodimerization of FKBP12 and FRB is one of the most commonly employed switches to conditionally control biological processes. We developed an optically activated rapamycin dimer that does not induce FKBP12-FRB dimerization until exposed to light, and applied it to control kinase, protease, and recombinase function.
Publication types
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Research Support, U.S. Gov't, Non-P.H.S.
MeSH terms
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Humans
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Molecular Conformation
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Optics and Photonics
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Photolysis / radiation effects*
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Protein Multimerization / radiation effects*
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Sirolimus / chemistry*
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Sirolimus / radiation effects*
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Tacrolimus Binding Protein 1A / chemistry*
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Ultraviolet Rays*
Substances
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Tacrolimus Binding Protein 1A
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Sirolimus