Abstract
We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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DNA, Catalytic / chemistry*
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DNA, Catalytic / metabolism*
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Fluorescence*
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Fluorescent Dyes / analysis
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Fluorescent Dyes / chemistry
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Fluorometry
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Graphite / chemistry*
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Mutation
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Oxides / chemistry*
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RNA / chemistry*
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RNA / genetics
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RNA / metabolism
Substances
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DNA, Catalytic
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Fluorescent Dyes
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Oxides
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RNA
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Graphite