Fluorescence-based detection of single-nucleotide changes in RNA using graphene oxide and DNAzyme

Chem Commun (Camb). 2015 Apr 4;51(26):5641-4. doi: 10.1039/c4cc09603g.

Abstract

We report a simple fluorometric method for detection of single-nucleotide changes in RNA using graphene oxide (GO) and RNA-cleaving DNAzyme. The fluorescent DNA probe (F-DNA) was annealed to RNA fragments generated by RNA cleavage with DNAzyme specific to mutant RNA. The F-DNA-RNA duplex attenuated the quenching of F-DNA fluorescence by GO.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • DNA, Catalytic / chemistry*
  • DNA, Catalytic / metabolism*
  • Fluorescence*
  • Fluorescent Dyes / analysis
  • Fluorescent Dyes / chemistry
  • Fluorometry
  • Graphite / chemistry*
  • Mutation
  • Oxides / chemistry*
  • RNA / chemistry*
  • RNA / genetics
  • RNA / metabolism

Substances

  • DNA, Catalytic
  • Fluorescent Dyes
  • Oxides
  • RNA
  • Graphite