Bestatin induces specific changes in Trypanosoma cruzi dipeptide pool

Antimicrob Agents Chemother. 2015 May;59(5):2921-5. doi: 10.1128/AAC.05046-14. Epub 2015 Feb 23.

Abstract

Proteases and peptidases in Trypanosoma cruzi are considered potential targets for antichagasic chemotherapy. We monitored changes in low-mass metabolites in T. cruzi epimastigotes treated with bestatin, a dipeptide metalloaminopeptidase inhibitor. After treatment, multiple dipeptides were shown to be increased, confirming in situ inhibition of the leucine aminopeptidase of T. cruzi (LAPTc) and probably other peptidases.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Aminopeptidases / antagonists & inhibitors
  • Dipeptides / metabolism*
  • Leucine / analogs & derivatives*
  • Leucine / pharmacology
  • Trypanosoma cruzi / drug effects
  • Trypanosoma cruzi / enzymology*
  • Trypanosoma cruzi / metabolism*

Substances

  • Dipeptides
  • Aminopeptidases
  • Leucine
  • ubenimex