Comparison of the expression of immunomodulatory factors in cultures of mesenchymal stromal cells from human extraembryonic tissues

A M Savilova; A V Zakharov; E A Metlyuk; Ya V Serdyuk; V N Veryasov; A M Krasnyi; D Yu Trofimov

doi:10.1007/s10517-015-2806-z

Comparison of the expression of immunomodulatory factors in cultures of mesenchymal stromal cells from human extraembryonic tissues

Bull Exp Biol Med. 2015 Feb;158(4):555-60. doi: 10.1007/s10517-015-2806-z. Epub 2015 Feb 24.

Authors

A M Savilova¹, A V Zakharov, E A Metlyuk, Ya V Serdyuk, V N Veryasov, A M Krasnyi, D Yu Trofimov

Affiliation

¹ V. I. Kulakov Research Center of Obstetrics, Gynecology, and Perinatology, Ministry of Health of the Russian Federation, Moscow, Russia, a_savilova@oparina4.ru.

PMID: 25705041
DOI: 10.1007/s10517-015-2806-z

Abstract

mRNA of genes (tgfβ, il6, il10, il1β, and vegf165) involved in cell proliferation, inflammatory, anti-inflammatory, and angiogenic processes were detected and quantified in cultures of mesenchymal stromal cells of different passages derived from human extraembryonic tissues (amniotic sac, umbilical cord, chorionic villi and trophoblast of the placenta). The concentrations of IL-10, IL-6, IL-1β, and TGFβ were measured.

Publication types

Comparative Study
Research Support, Non-U.S. Gov't

MeSH terms

Cells, Cultured
Extraembryonic Membranes / cytology*
Humans
Immunologic Factors / metabolism*
Interleukin-10 / metabolism
Interleukin-1beta / metabolism
Interleukin-6 / metabolism
Mesenchymal Stem Cells / immunology*
RNA, Messenger / metabolism*
Real-Time Polymerase Chain Reaction
Spectrophotometry
Statistics, Nonparametric
Transforming Growth Factor beta / metabolism
Trophoblasts / cytology*
Umbilical Cord / cytology*

Substances

Immunologic Factors
Interleukin-1beta
Interleukin-6
RNA, Messenger
Transforming Growth Factor beta
Interleukin-10