Successful implantation of autologous valved conduits with self-expanding stent (stent-biovalve) within the pulmonary artery in beagle dogs

Marina Funayama; Yuichi Matsui; Tsutomu Tajikawa; Taiki Sasagawa; Yuta Saito; Shoko Sagishima; Takeshi Mizuno; Masashi Mizuno; Kayoko Harada; Shuhei Uchida; Asako Shinoda; Ryosuke Iwai; Yasuhide Nakayama; Masami Uechi

doi:10.1016/j.jvc.2014.12.003

Successful implantation of autologous valved conduits with self-expanding stent (stent-biovalve) within the pulmonary artery in beagle dogs

J Vet Cardiol. 2015 Mar;17(1):54-61. doi: 10.1016/j.jvc.2014.12.003. Epub 2015 Feb 16.

Authors

Affiliations

¹ Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan; College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
² Department of Mechanical Systems Engineering, Kansai University, 3-3-35 Yamate-cho, Suita, Osaka 564-8680, Japan.
³ College of Bioresource Sciences, Nihon University, 1866 Kameino, Fujisawa, Kanagawa 252-0880, Japan.
⁴ Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan; Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan.
⁵ Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan.
⁶ Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan.
⁷ Division of Medical Engineering and Materials, National Cerebral and Cardiovascular Center Research Institute, 5-7-1 Fujishiro-dai, Suita, Osaka 565-8565, Japan; Japan Animal Specialty Medical Institute Inc., 2-7-3, Nakagawa, Tsuduki-ku, Yokohama, Kanagawa 224-0001, Japan. Electronic address: uechi.masami@cardiovets.jp.

PMID: 25697493
DOI: 10.1016/j.jvc.2014.12.003

Abstract

Objectives: To evaluate the functionality of an autologous heart valve with stent (Stent-biovalve or SBV) after implantation in the pulmonic valve position in beagle dogs.

Animals: Five beagle dogs.

Methods: A mold with an aperture of a tri-leaflet structure was constructed from a pair of concave and convex rods to which a nitinol (NiTi) stent was mounted. This mold was embedded in a dorsal subcutaneous pouch in beagle dogs for 4 weeks. At the time of the removal, the surfaces of the molds were completely covered with connective tissues, tri-leaflet valves were formed and the NiTi stent was tightly connected to the structure.

Results: The mean burst strength of the SBV leaflet was 2710 mmHg (range 2280-3116 mmHg), which was approximately equal to that of the native pulmonic valve leaflet. After implantation in the pulmonary position, the SBV showed good functionality as a pulmonic valve. At 84 days after implantation, the SBV was replaced with autologous fibroblasts and collagenous tissues, and showed organization similar to that of native heart valves.

Conclusion: Stent-Biovalves achieved good valvular function with laminar flow in the pulmonic valve position of beagle dogs.

Keywords: Heart valve replacement; Pulmonic stenosis; Regenerative medicine; Self-expandable stent; Tissue engineering.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Bioengineering / methods*
Biomechanical Phenomena
Bioprosthesis*
Connective Tissue
Dogs*
Materials Testing*
Stents / veterinary*