Expression of the novel hepatitis C virus core+1/ARF protein in the context of JFH1-based replicons

Ioly Kotta-Loizou; Ioannis Karakasiliotis; Niki Vassilaki; Panagiotis Sakellariou; Ralf Bartenschlager; Penelope Mavromara

doi:10.1128/JVI.02351-14

Expression of the novel hepatitis C virus core+1/ARF protein in the context of JFH1-based replicons

J Virol. 2015 May;89(9):5164-70. doi: 10.1128/JVI.02351-14. Epub 2015 Feb 18.

Authors

Ioly Kotta-Loizou¹, Ioannis Karakasiliotis¹, Niki Vassilaki¹, Panagiotis Sakellariou¹, Ralf Bartenschlager², Penelope Mavromara³

Affiliations

¹ Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece.
² Department of Infectious Diseases, Molecular Virology, University Hospital Heidelberg, Heidelberg, Germany.
³ Molecular Virology Laboratory, Hellenic Pasteur Institute, Athens, Greece Department of Molecular Biology and Genetics, Democritus University of Thrace, Thrace, Greece penelopm@pasteur.gr.

Abstract

Hepatitis C virus contains a second open reading frame within the core gene, designated core+1/ARF. Here we demonstrate for the first time expression of core+1/ARF protein in the context of a bicistronic JFH1-based replicon and report the production of two isoforms, core+1/L (long) and core+1/S (short), with different kinetics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Cell Line
Gene Expression Profiling
Gene Expression*
Hepacivirus / genetics*
Hepatocytes / virology*
Humans
Protein Isoforms / biosynthesis
Protein Isoforms / genetics
Replicon
Viral Core Proteins / biosynthesis*
Viral Core Proteins / genetics

Substances

Protein Isoforms
Viral Core Proteins
hepatitis C protein F, Hepatitis C virus