Direct interactions in the recognition between the environmental estrogen bisphenol AF and human serum albumin

J Mol Recognit. 2015 Aug;28(8):459-66. doi: 10.1002/jmr.2463. Epub 2015 Feb 19.

Abstract

Bisphenol AF (BPAF) was used as a model compound to investigate the binding mechanism between the endocrine disrupting compound and human serum albumin (HSA) using multispectroscopic techniques and molecular modeling method at the protein level. The results indicated that BPAF was indeed bound to HSA and located in the hydrophobic pocket of HSA on subdomain IIA through hydrogen bond and van der Waals interactions. The fluorescence quenching data showed that the binding of BPAF and HSA quenched the intrinsic fluorescence of HSA, and the static quenching constants were acquired.

Keywords: Bisphenol AF; Human serum albumin; Interaction; Multi-spectroscopic techniques.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Benzhydryl Compounds / chemistry*
  • Benzhydryl Compounds / toxicity*
  • Binding Sites
  • Endocrine Disruptors / chemistry*
  • Endocrine Disruptors / toxicity
  • Estrogens / chemistry*
  • Estrogens / toxicity*
  • Humans
  • Hydrogen Bonding
  • Models, Molecular
  • Phenols / chemistry*
  • Phenols / toxicity*
  • Protein Binding
  • Protein Structure, Secondary
  • Serum Albumin / chemistry*
  • Spectrometry, Fluorescence
  • Thermodynamics

Substances

  • Benzhydryl Compounds
  • Endocrine Disruptors
  • Estrogens
  • Phenols
  • Serum Albumin
  • 4,4'-hexafluorisopropylidene diphenol