Numerous studies have indicated that rising ozone (O3) in the troposphere significantly decreased the photosynthesis and the activity of Rubisco enzyme. So it can be inferred that the N uptake and distribution within the plants could be affected by elevated O3. In this study, ten greening woody species, widely distributed in subtropical China, were exposed to charcoal-filtered air (CF, less than 20 nL · L(-1)) and elevated O3 (E-O3, mean concentration of 150 nL · L(-1)) in open top chambers. The results showed that E-O3 significantly reduced the leaves biomass in Liquidamba formosana by 20.9%, the stem biomass in Liriodendron chinense by 21.4%, the root biomass in L. formosana and L. chinense by 24.2% and 32.5%, respectively. E-O3 significantly affected the N concentration in the stem but not those in leaves and root. The N uptakes in the whole tree (Nlu), the leaves and the root were significantly affected by E-O3. Compared to CF, E-O3 significantly reduced the Nlu in L. chinense by 28.4% and Schima superba by 22.7% but significantly increased the Nlu in Neolitsea sericea by 15.5%. Elevated O3 concentration had no significant influence on N distribution within the plants across the selected 10 tree species.