Somatic reprogramming to generate induced pluripotent stem cells, or iPSC, is a powerful tool in developmental biology, disease modeling, and regenerative medicine. microRNAs have been shown to regulate many key pathways in iPSC induction. Here we describe a microRNA mimic enhanced somatic reprogramming process starting from mouse embryonic fibroblast isolation to iPSC induction to colony derivation and characterization.
Keywords: Differentiation; Embryoid body; Induced pluripotent stem cell; MicroRNA; Mouse embryonic fibroblasts; Somatic reprogramming; Teratoma; Transfection.