Near infrared (NIR) laser mediated surface activation of graphene oxide nanoflakes for efficient antibacterial, antifungal and wound healing treatment

M Shahnawaz Khan; Hani Nasser Abdelhamid; Hui-Fen Wu

doi:10.1016/j.colsurfb.2014.12.049

Near infrared (NIR) laser mediated surface activation of graphene oxide nanoflakes for efficient antibacterial, antifungal and wound healing treatment

Colloids Surf B Biointerfaces. 2015 Mar 1:127:281-91. doi: 10.1016/j.colsurfb.2014.12.049. Epub 2015 Jan 5.

Authors

M Shahnawaz Khan¹, Hani Nasser Abdelhamid², Hui-Fen Wu³

Affiliations

¹ Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan.
² Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan; Department of Chemistry, Assuit University, Assuit 71515, Egypt.
³ Department of Chemistry, National Sun Yat-Sen University, Kaohsiung, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan; School of Pharmacy, College of Pharmacy, Kaohsiung Medical University, Kaohsiung 806, Taiwan; Doctoral Degree Program in Marine Biotechnology, National Sun Yat-Sen University, Kaohsiung 80424, Taiwan; Center for Nanoscience and Nanotechnology, National Sun Yat-Sen University, 70, Lien-Hai Road, Kaohsiung 80424, Taiwan; Institute of Medical Science and Technology, National Sun Yat-Sen University, 80424, Taiwan. Electronic address: hwu@faculty.nsysu.edu.tw.

PMID: 25687099
DOI: 10.1016/j.colsurfb.2014.12.049

Abstract

Photothermal treatment of graphene oxide (GO) for antibacterial, antifungal and controlling the wound infection treatment using near infrared laser (NIR, Nd-YAG (λ=1064 nm) were reported. Various pathogenic bacteria (Pseudomonas aeruginosa, Staphylococcus aureus) and fungi (Saccharomyces cerevisiae and Candida utilis) were investigated. The cytotoxicity was measured using the proteomic analysis by matrix assisted laser desorption/ionization mass spectrometry (MALDI-MS), optical density (OD600), standard microdilution procedures, transmission electron microscopy (TEM) and epifluorescence microscopy. The laser mediated the surface activation of GO offer high efficiency for antifungal and antibacterial. Wide broad cells with various instruments approved that graphene oxide is promising material for nanomedicine in the near future.

Keywords: Antibacterial; Antifungal; Graphene oxide; Photothermal treatment; Proteomics.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Anti-Bacterial Agents / pharmacology*
Antifungal Agents / pharmacology*
Cell Death / drug effects
Colony Count, Microbial
Fungi / drug effects
Graphite / pharmacology*
Lasers, Solid-State*
Male
Mice
Microbial Sensitivity Tests
Microscopy, Fluorescence
Nanoparticles / chemistry*
Nanoparticles / ultrastructure
Oxides / pharmacology*
Proteomics
Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization
Spectrometry, X-Ray Emission
Spectrophotometry, Ultraviolet
Spectroscopy, Fourier Transform Infrared
Spectroscopy, Near-Infrared*
Spectrum Analysis, Raman
Staphylococcus aureus / drug effects
Staphylococcus aureus / ultrastructure
Wound Healing / drug effects*

Substances

Anti-Bacterial Agents
Antifungal Agents
Oxides
Graphite