Identification of five Shiga toxin-producing Escherichia coli genes by Luminex microbead-based suspension array

Insook Son; Rachel Binet; Andrew Lin; Thomas S Hammack; Julie A Kase

doi:10.1016/j.mimet.2015.02.008

Identification of five Shiga toxin-producing Escherichia coli genes by Luminex microbead-based suspension array

J Microbiol Methods. 2015 Apr:111:108-10. doi: 10.1016/j.mimet.2015.02.008. Epub 2015 Feb 14.

Authors

Insook Son¹, Rachel Binet², Andrew Lin³, Thomas S Hammack⁴, Julie A Kase⁵

Affiliations

¹ U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address: insook.son@fda.hhs.gov.
² U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address: rachel.binet@fda.hhs.gov.
³ U.S. Food and Drug Administration, San Francisco Laboratory, Alameda, CA 94502, USA. Electronic address: andrew.lin@fda.hhs.gov.
⁴ U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address: thomas.hammack@fda.hhs.gov.
⁵ U.S. Food & Drug Administration, Center for Food Safety and Applied Nutrition, Office of Regulatory Science, College Park, MD 20740, USA. Electronic address: julie.kase@fda.hhs.gov.

PMID: 25686714
DOI: 10.1016/j.mimet.2015.02.008

Abstract

To rapidly identify the presence of potentially virulent O157:H7 and non-O157 Shiga toxin-producing Escherichia coli (STEC), a PCR-based Luminex suspension assay was developed to detect the genes coding for four virulence factors (stx1, stx2, eae, and ehxA) plus the O157:H7-specific +93 uidA single nucleotide polymorphism.

Published by Elsevier B.V.

MeSH terms

Adhesins, Bacterial / genetics
Escherichia coli O157 / genetics*
Escherichia coli O157 / isolation & purification
Escherichia coli Proteins / genetics
Genes, Bacterial*
Hemolysin Proteins / genetics
Microspheres
Polymerase Chain Reaction / methods*
Polymorphism, Single Nucleotide
Shiga Toxin 1 / genetics
Shiga Toxin 2 / genetics
Shiga-Toxigenic Escherichia coli / genetics*
Shiga-Toxigenic Escherichia coli / isolation & purification
Virulence Factors / genetics*

Substances

Adhesins, Bacterial
Escherichia coli Proteins
Hemolysin Proteins
Hlya protein, E coli
Shiga Toxin 1
Shiga Toxin 2
Virulence Factors
eaeA protein, E coli