Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus

L Mirimin; R Roodt-Wilding

doi:10.1111/jfb.12639

Testing and validating a modified CTAB DNA extraction method to enable molecular parentage analysis of fertilized eggs and larvae of an emerging South African aquaculture species, the dusky kob Argyrosomus japonicus

J Fish Biol. 2015 Mar;86(3):1218-23. doi: 10.1111/jfb.12639. Epub 2015 Feb 12.

Authors

L Mirimin¹, R Roodt-Wilding

Affiliation

¹ Molecular Breeding and Biodiversity Research Group, Department of Genetics, Stellenbosch University, JC Smuts Building, van der Bijl Street, Stellenbosch 7600, South Africa; Marine and Freshwater Research Centre, Galway-Mayo Institute of Technology, Dublin Road, Galway, Ireland.

PMID: 25683971
DOI: 10.1111/jfb.12639

Abstract

This study describes the successful implementation of a modified cetyltrimethyl ammonium bromide (CTAB) protocol to isolate genomic DNA and amplify 14 microsatellite markers from fertilized eggs and larvae of an emerging South African farmed marine fish species, the dusky kob Argyrosomus japonicus. To test and validate the efficiency of this method, genetic data were utilized to resolve parentage and kinship of first-generation (F1) offspring produced in mass-spawning events of wild broodstock fish in a commercial hatchery.

Keywords: F1; Kabeljou; first generation; genomic DNA isolation; microsatellite loci; pedigree analyses.

Publication types

Research Support, Non-U.S. Gov't
Validation Study

MeSH terms

Animals
Aquaculture
DNA / genetics
DNA / isolation & purification*
Female
Larva / genetics
Male
Microsatellite Repeats*
Perciformes / genetics*
Sequence Analysis, DNA
Zygote

Substances

DNA