Detection of drug susceptibility in rapidly growing mycobacteria by resazurin broth microdilution assay

Aline Lemes Castilho; Katiany Rizzieri Caleffi-Ferracioli; Pedro Henrique Canezin; Vera Lúcia Dias Siqueira; Regiane Bertin de Lima Scodro; Rosilene Fressatti Cardoso

doi:10.1016/j.mimet.2015.02.007

Detection of drug susceptibility in rapidly growing mycobacteria by resazurin broth microdilution assay

J Microbiol Methods. 2015 Apr:111:119-21. doi: 10.1016/j.mimet.2015.02.007. Epub 2015 Feb 12.

Authors

Aline Lemes Castilho¹, Katiany Rizzieri Caleffi-Ferracioli², Pedro Henrique Canezin¹, Vera Lúcia Dias Siqueira², Regiane Bertin de Lima Scodro², Rosilene Fressatti Cardoso³

Affiliations

¹ Postgraduate Program in Health Science, State University of Maringá, Paraná, Brazil.
² Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil.
³ Laboratory of Medical Bacteriology, Department of Clinical Analysis and Biomedicine, State University of Maringá, Paraná, Brazil. Electronic address: rfressatticardoso@gmail.com.

PMID: 25683207
DOI: 10.1016/j.mimet.2015.02.007

Abstract

The susceptibility of rapidly growing mycobacteria (RGM) to linezolid and ciprofloxacin was evaluated by using resazurin as a growth indicator. The assay with resazurin supplemented medium performed as well as its addition to the medium at reading time and was efficient for the determination for the susceptibility profile in RGM.

Keywords: Ciprofloxacin; Linezolid; Rapidly growing mycobacteria; Resazurin.

MeSH terms

Anti-Bacterial Agents / pharmacology*
Ciprofloxacin / pharmacology
Linezolid / pharmacology
Microbial Sensitivity Tests / methods*
Nontuberculous Mycobacteria / drug effects*
Nontuberculous Mycobacteria / growth & development*
Nontuberculous Mycobacteria / metabolism
Oxazines / metabolism*
Xanthenes / metabolism*

Substances

Anti-Bacterial Agents
Oxazines
Xanthenes
resazurin
Ciprofloxacin
Linezolid