Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells

Xibao Shi; Yongzhe Chang; Xiaozhuan Zhang; Li Wang; Chunxi Li; Kai Jiang; Jing Chen; Chao Wang; Ruiguang Deng; Jianming Fan; Gaiping Zhang

doi:10.1016/j.rvsc.2015.01.015

Small interfering RNA targeting nonstructural protein1 α (nsp1α) of porcine reproductive and respiratory syndrome virus (PRRSV) can reduce the replication of PRRSV in MARC-145 cells

Res Vet Sci. 2015 Apr:99:215-7. doi: 10.1016/j.rvsc.2015.01.015. Epub 2015 Jan 30.

Authors

Xibao Shi¹, Yongzhe Chang², Xiaozhuan Zhang³, Li Wang⁴, Chunxi Li⁵, Kai Jiang⁶, Jing Chen⁴, Chao Wang⁴, Ruiguang Deng⁴, Jianming Fan⁷, Gaiping Zhang⁸

Affiliations

¹ College of Life Sciences, Henan Normal University, Xinxiang 453007, China; College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
² College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
³ College of Life Sciences, Henan Normal University, Xinxiang 453007, China; College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
⁴ Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China.
⁵ College of Life Sciences, Henan Normal University, Xinxiang 453007, China.
⁶ College of Chemistry and Chemical Engineering, Henan Normal University, Xinxiang 453007, China.
⁷ The Laboratory of Toxicology, College of Public Health, Zhengzhou University, Zhengzhou 450001, China.
⁸ College of Veterinary Medicine and Animal Science, Henan Agricultural University, Zhengzhou, Henan 450002, China; Key Laboratory of Animal Immunology of the Ministry of Agriculture, Henan Provincial Key Laboratory of Animal Immunology, Henan Academy of Agricultural Sciences, Zhengzhou, Henan 450002, China. Electronic address: zhanggaiping2003@163.com.

Abstract

Porcine reproductive and respiratory syndrome virus (PRRSV) is one of the most economically devastating and pandemic diseases of swine, which is poorly controlled by current methods. The inhibition of specific genes by small interfering RNA (siRNA) has been proven to be a potential therapeutic strategy against viral infection. Previous studies have indicated that the nonstructural protein 1α (nsp1α) of PRRSV may take an important role in virulence of PRRSV. The present work was involved to explore the effect of siRNA targeting nsp1α on the replication of PRRSV in MARC-145 cells, and the results showed that over-expression of nsp1α enhanced the replication of PRRSV and that siRNAs specifically targeting nsp1α significantly inhibited the replication of PRRSV in MARC-145 cells. In conclusion, this work indicated that nsp1α may be a viral pathogenicity factor of PRRSV and that siRNAs specifically targeting nsp1α may be a new strategy to control PRRSV in the future.

Keywords: PRRSV; Small interfering RNA; nsp1α.

Publication types

Research Support, Non-U.S. Gov't

MeSH terms

Animals
Cell Line
Haplorhini
Porcine respiratory and reproductive syndrome virus / genetics
Porcine respiratory and reproductive syndrome virus / physiology*
RNA, Small Interfering / genetics*
RNA, Small Interfering / metabolism
Viral Nonstructural Proteins / genetics*
Viral Nonstructural Proteins / metabolism
Virus Replication*

Substances

RNA, Small Interfering
Viral Nonstructural Proteins