On-column enrichment and surface-enhanced Raman scattering detection in nanoparticles functionalized porous capillary monolith

Spectrochim Acta A Mol Biomol Spectrosc. 2015 Apr 15:141:244-51. doi: 10.1016/j.saa.2015.01.044. Epub 2015 Jan 30.

Abstract

A monolithic column functionalized with gold nanoparticles (GNPs) was designed to provide ultrasensitive detection with surface-enhanced Raman scattering (SERS). The monolithic column based on poly (glycidyl methacrylate-co-ethylene dimethacrylate) (GMA-co-EDMA) was served as the enrichment sorbent to concentrate GNPs and targets. We found that 60 nm GNPs-functionalized monolithic columns demonstrated the best SERS enhancement, and the lowest detectable concentration for PATP and CV could be achieved at 10(-7) and 10(-11) M, respectively. Moreover, the columns exhibit a good reproducibility in both spot-to-spot (∼10%) and batch-to-batch (∼15%). The SERS monolithic column with a high sensitivity and reproducibility has a great potential in the field-based rapid detection of targets in complex real-world samples.

Keywords: Gold nanoparticles; On-column enrichment; Porous capillary monolith; SERS.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Adsorption
  • Aniline Compounds / chemistry
  • Gold / chemistry
  • Metal Nanoparticles / chemistry*
  • Metal Nanoparticles / ultrastructure
  • Methylmethacrylates / chemistry
  • Particle Size
  • Porosity
  • Spectrophotometry, Ultraviolet
  • Spectrum Analysis, Raman*
  • Sulfhydryl Compounds / chemistry

Substances

  • Aniline Compounds
  • Methylmethacrylates
  • Sulfhydryl Compounds
  • GMA-EDMA
  • Gold
  • 4-aminothiophenol