Fast bi-exponential fluorescence lifetime imaging analysis methods

Opt Lett. 2015 Feb 1;40(3):336-9. doi: 10.1364/OL.40.000336.

Abstract

A new hardware-friendly bi-exponential fluorescence lifetime imaging (FLIM) algorithm has been proposed. Compared to conventional FLIM software, the proposed algorithms are noniterative offering direct calculation of lifetimes and therefore suitable for real-time applications. They are applicable to single-channel or 2D multichannel time-correlated single-photon counting (TCSPC) systems. The proposed methods have been tested on both synthesized and realistic FLIM data, and we have compared their performances with other recently proposed nonfitting bi-exponential techniques showing promising applications in future massive solid-state TCSPC imagers.

Publication types

  • Research Support, Non-U.S. Gov't

MeSH terms

  • Algorithms
  • Image Processing, Computer-Assisted
  • Microscopy, Fluorescence / methods*