Abstract
Selenocysteine is a valuable component of both natural selenoproteins and designer biocatalysts; however the availability of such proteins is hampered by technical limitations. Here we report the first general strategy for the production of selenoproteins via genetically-encoded incorporation of a synthetic photocaged selenocysteine residue in yeast cells, and provide examples of light-controlled protein dimerization and targeted covalent labeling in vitro.
Publication types
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Research Support, Non-U.S. Gov't
MeSH terms
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Benzyl Compounds / chemistry
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Benzyl Compounds / metabolism
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Green Fluorescent Proteins / genetics
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Green Fluorescent Proteins / metabolism
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Recombinant Proteins / genetics
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Recombinant Proteins / metabolism
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Selenocysteine / chemistry
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Selenocysteine / metabolism*
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Selenoproteins / genetics
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Selenoproteins / metabolism*
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Yeasts / genetics
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Yeasts / metabolism*
Substances
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Benzyl Compounds
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Recombinant Proteins
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Selenoproteins
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enhanced green fluorescent protein
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Selenocysteine
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Green Fluorescent Proteins