Vertical Nanowire Electrode Arrays as Novel Electrochemical Label-Free Immunosensors

Babak Nasr; Gursharan Chana; Ting Ting Lee; Thanh Nguyen; Chathurika Abeyrathne; Giovanna M D'Abaco; Mirella Dottori; Efstratios Skafidas

doi:10.1002/smll.201403540

Vertical Nanowire Electrode Arrays as Novel Electrochemical Label-Free Immunosensors

Small. 2015 Jun 24;11(24):2862-8. doi: 10.1002/smll.201403540. Epub 2015 Feb 11.

Authors

Babak Nasr^{1

2}, Gursharan Chana^{1

3}, Ting Ting Lee^{1

3}, Thanh Nguyen^{1

2}, Chathurika Abeyrathne^{1

2}, Giovanna M D'Abaco^{1

2}, Mirella Dottori^{1

2}, Efstratios Skafidas^{1

2

3}

Affiliations

¹ Centre for Neural Engineering, The University of Melbourne, Parkville, Victoria, 3010, Australia.
² Department of Electrical and Electronic Engineering, Melbourne School of Engineering, The University of Melbourne, Victoria, 3010, Australia.
³ Department of Psychiatry, Royal Melbourne Hospital, The University of Melbourne, Victoria, 3050, Australia.

PMID: 25677788
DOI: 10.1002/smll.201403540

Abstract

A new method for the fabrication of a label-free electrochemical immunosensor based on vertical nanowires (VNWs) is proposed. The VNWs are functionalized to detect antibodies against a major astrocytic structural protein component, glial fibrillary acidic protein (GFAP). It is revealed that the interaction of GFAP-antibody with functionalized VNWs leads to a clear change in device conductance and the corresponding capacitance.

Keywords: biosensors; electrode arrays; label-free detection; nanowires; silicon.

MeSH terms

Antibodies / metabolism
Biosensing Techniques / instrumentation*
Electrochemical Techniques / instrumentation*
Electrodes
Fluorescent Antibody Technique
Glial Fibrillary Acidic Protein / metabolism
Humans
Immunoassay / instrumentation*
Nanowires*
Protein Binding

Substances

Antibodies
Glial Fibrillary Acidic Protein