Esterases as stereoselective biocatalysts

Diego Romano; Francesco Bonomi; Marcos Carlos de Mattos; Thiago de Sousa Fonseca; Maria da Conceição Ferreira de Oliveira; Francesco Molinari

doi:10.1016/j.biotechadv.2015.01.006

Esterases as stereoselective biocatalysts

Biotechnol Adv. 2015 Sep-Oct;33(5):547-65. doi: 10.1016/j.biotechadv.2015.01.006. Epub 2015 Feb 10.

Authors

Diego Romano¹, Francesco Bonomi¹, Marcos Carlos de Mattos², Thiago de Sousa Fonseca², Maria da Conceição Ferreira de Oliveira², Francesco Molinari¹

Affiliations

¹ Department of Food, Environmental and Nutritional Sciences (DEFENS), University of Milan, Via Mangiagalli 25, 20133 Milan, Italy.
² Department of Organic and Inorganic Chemistry, Federal University of Ceará, Campus do Pici, Postal Box 6044, 60455-970 Fortaleza, Ceará, Brazil.

PMID: 25677731
DOI: 10.1016/j.biotechadv.2015.01.006

Abstract

Non-lypolitic esterases are carboxylester hydrolases with preference for the hydrolysis of water-soluble esters bearing short-chain acyl residues. The potential of esterases as enantioselective biocatalysts has enlarged in the last few years due to the progresses achieved in different areas, such as screening methodologies, overproduction of recombinant esterases, structural information useful for understanding the rational behind enantioselectivity, and efficient methods in protein engineering. Contributions of these complementary know-hows to the development of new robust enantioselective esterases are critically discussed in this review.

Keywords: Biocatalysis; Carboxylester hydrolases; Enantioselectivity; Esterases; Kinetic resolution; Medium engineering; Protein engineering.

Publication types

Research Support, Non-U.S. Gov't
Review

MeSH terms

Biotechnology
Esterases*
Protein Engineering
Stereoisomerism

Substances

Esterases