Clickable co-substrate: A tri-functional 5'-γ-ferrocenyl adenosine triphosphate (Fc-ATP) derivative containing a clickable site was synthesized. This compound is an effective co-substrate in kinase-catalyzed phosphorylation reactions, which can be detected by both electrochemical and immunoassay detection methods. The clickable reaction site makes direct modification possible, which greatly expands its application.
Keywords: click chemistry; ferrocenes; peptides; phosphorylation; protein kinases.
© 2015 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim.